This month EAACI's Thematic Month Section focuses on Allergy Diagnosis. In modern allergy diagnosis, the responsiveness to allergen suggested by the clinical history of an allergic patient should be confirmed by an ex vivo or in vitro test. These laboratory tests are the realm of the Interest Group for Allergy Diagnosis (IGAD). The commonly used in vitro test is determination of specific IgE, whereas the ex vivo test may be histamine release or up regulation of surface molecules CD63 or CD203c on basophil granulocytes, known as the basophil activation test, BAT.
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